ATP and other Luminescence Reagents
ATP Reagent SL especially developed for the assay of mithocondrial ATP metabolism. The reagent has a decay of ATP and light emission of less than 1% per minute. The stable light allows manual handling and you can use a simpler luminometer. The detection limit is 10^-15 mole ATP, making the reagent suitable for assays of mithocondrial ATP production rate down to the detection limit and for other continous monitoring of enzymatic reactions where the magnesium ion concentration has to be adjusted.
Luciferin is a substrate for luciferase together with ATP. Substrate is used with 51-101 ATP Substrate and is intended for in vitro studies of firefly luciferase. The reagent contains D-Luciferin and luciferase- activators and stabilisers to obtain a stable light (less than 2% decay per minute). 61-101 ATP Substrate should be reconstituted in 10 mL Tris-EDTA buffer.
D-luciferin is used for in-vivo and in-vitro studies of firefly luciferase and high quality luciferin is a vital component of BioThemas kits.
Delivery form: Lyophilised reagent in a glass vial, sealed under argon
Reconstitution: In 23-101 Diluent C. A further 5 fold dilution in Tris-EDTA buffer or other suitable buffer is recomended, for maximum performance.
Number of assays: Up to 250 analyses in a 96 well microplate format or 50 analyses in cuvette format.
Linear range: From detection limit to 10^-15 mole ATP, depending on the linearity of the luminometer.
Shelf life: 2 years from manufacture. Reconstituted reagent should be used within a week.