Cell Proliferation and Cytotoxicity Kits
For cell proliferation & cytotoxicity
The amount of ATP reflects the intracellular volume as its central role in metabolism makes it necessary for all cells to maintain a constant intracellular concentration. The amount of ATP is similar in all cells, about 1 attomole for bacterial cells and one femtomole for mammalian cells. If one measures the amount of ATP per sample can, one can calculate the number of cells per mL.
To obtain a stable light, all the ATPases (ATP degrading enzymes) have to be completely inhibited. For most samples the Cellular ATP Kit HTS is suitable, and in addition the kit is suitable for High Throughput Screening (HTS). However with some types of cells, such as nerve cells at high cell densities, the ATPase inhibitor in the Cellular ATP Kit HTS may not be strong enough to completely inhibit the ATPase. In those cases 188-441 Cell Viablity Kit SL might be an alternative, as it contains a stronger ATPase inhibitor.
55-050 Cellular ATP Kit HTS is intended for use with mammalian cell samples. It is an easy-to-use kit where the lysing buffer, an ATPase inhibitor, and the ATP Reagent are combined in one reagent. The light emission is extremly stable, providing that all ATPases in the sample have been completly inhibited. That makes the 155-050 Cellular ATP Kit HTS suitable for high throughput screening.
In samples with a high density of ATPases, e.g. nerve cells, the ATPase inhibitor in the Cellular ATP Kit might not completly inhibit the ATPases. This will result in a slight decay of light. In such cases, a luminometer with dispensers could be used. Alternatively use 188-441 Cell Viability Kit SL which contains a stronger ATPase inhibitor.