A crucial step in the ATP assay is the extraction of the ATP from the cells, when the cells are opened and the ATPases in the cell neutralized. The process has to be very fast, since if the ATPases are not neutralized immediately they will start degrading the ATP.
If the extractant is not neutralized after use it will degrade the luciferase in the ATP reagents when added. This is avoided by reconstituting the ATP Reagents in Diluent B as the diluent contains components to neutralize the extractant.
Extract B/S used for the extraction of ATP from bacterial and mammalian cells
Cell Lysing Reagent is intended for the extraction of ATP from mammalian cells. It contains a milder detergent than Extractant B/S and will not affect bacterial cells. If Cell Lysing Reagent is reconstituted in 71-101 ATP Eliminating Reagent extracellular ATP will be eliminated as well, for cases when only bacterial ATP is of interest.
Lysing Diluent is used to release ATP from mammalian cells. It contains an ATPase inhibitor, to avoid degradation of the extracellular ATP after the cells have been opened.