X-CLARITY™ systems and reagents standardise, simplify, and accelerate each step of the tissue clearing process.
Using CLARITY, chemically preserved (fixed) tissues are embedded in a hydrogel matrix. Then lipids are actively extracted using electrophoresis to create a stable and optically transparent tissue-hydrogel hybrid that is chemically accessible for multiple rounds of antibody labelling and imaging. This method ensures that native cytoarchitecture remains intact and even endogenous fluorescence proteins are preserved for robust fluorescence downstream imaging.
Thanks to advancements in optical technology, the quality of cell imaging data has increased dramatically since the conception of the microscope. User experience, however, has not improved at the same rate. Complex alignment processes, long setup procedures, and time consuming adjustments require extensive training and expertise to master.
LUNA-II™ - next generation of automated cell counting with unmatched speed, accuracy and measurement consistency
The LUNA-II™ builds upon the success of LUNA™ by adding a novel autofocus system and newly optimised optics and also offers low running costs, ease of use and reliable data..
The integrated software accurately detects live/dead cells and discriminates the count from cell debris. Individual cells detected within cell clusters are also successfully counted by the LUNA-II™’s sophisticated de-clustering algorithm.
A fixed tissue sample is incubated in the X-CLARITY™ Hydrogel Solution Kit to allow hydrogel monomers to diffuse uniformly throughout the sample. Once the solution has permeated the tissue, the sample is placed in the X-CLARITY™ Polymerisation System. Monomers polymerise in the anaerobic environment, covalently linking the biomolecules to a sturdy hydrogel network, preserving molecular information and structural integrity.
LUNA reusable counting slides
The LUNA range of automated cell counters offer market-leading low running costs - which can now be reduced even further with the LUNA reusable counting slide. This allows you to combine the consistency of automated counting with the low running costs of a traditional haemocytometer: