Rapid Microbiology Kits
Microbial ATP Kit HS is intended for determination of intracellular ATP in bacterial cells. The Microbial ATP Kit is used when you have bacterial samples that are contaminated by extracellular ATP as well as mammalian cells. The kit contains ATP Eliminating Reagent and Cell Lysis Buffer for rapid and easy degradation of the non-bacterial ATP.
Intracellular ATP Kit HS is intended for determination of intracellular ATP in bacterial cells. The Intracellular ATP Kit is suitable if you for example want to determine ATP in a pure bacterial culture free of mammalian cells and extracellular ATP.
ATP Biomass Kit HS is intended for determination of the total ( both extracellular and intracellular) amount of ATP in a sample. The ATP Biomass Kit is suitable for virtually all types of samples.
The HS reagent used has a high sensitivity, making it possible to detect down to 5 bacterial cells ( depending on the sensitivity of the luminometer). The high sensitivity is due to the high luciferase activity in the reagent, which has the extra advantage that if the reagent is contaminated during reconstitution the ATP background will be degraded by one order of magnitude every 38 min. After mixing the sample and ATP Reagent HS the light will decay with approx. 6%/min. This is slow enough to allow manual mixing, if a luminometer with dispensers is not available.
Please note that the number of bacterial cells and CFU is not the same, since one CFU might consist of several bacterial cells. If the detection limit is not adequate it is possible to concentrate the sample by microfiltration. This will remove almost all extracellular ATP.
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