Reporter Gene Assays
Luciferases are enzymes that emit light. The luciferase BioThema uses is originally derived from the firefly. Assay Kit is based on that the firefly luciferase luc gene is one of the most commonly used reporter genes and allows the monitoring of promoter activity in the control of gene expression.
It has a number of advantages and is an interesting option to CAT assays and non-luminescent reporter gene assays:
1) Firefly luciferase is not present in normal cells.
2) The assay is simple and easy to perform.
3) You can use both simple, manual, luminometers as well as fully automatic microplate luminometers.
4) It is suitable for HTS.
The kit contains reagents for 800 assays. If you want to perform the assay as an HTS, the 484-102 LAS HTS Kit is suitable if you have a luminometer without dispensers and 484-103 LAS HTS is suitable if you have a luminometer with dispensers.