|Reduces the complexity of RNA (cDNA) or protein samples|
|Allows construction of DNA libraries with multiple insert sizes for improved assembly|
|Allows sequencing of valuable DNA and retrieving sample for later study|
Collect 12 Contiguous Fractions from a Single Sample
The SageELF is an electrophoresis system that separates DNA or protein samples by size, and then fractionates the whole sample, or section of sample, into 12 fractions. The system is equipped with pulsed-field electrophoresis for resolving large DNA.
Fractionation ranges are estimated and adjusted in software, and fractions are collected in buffer. One sample is fractionated on a single precast agarose cassette, and one or two cassettes may be processed at one time.
Key Downstream Technologies:
- Proteomics and mass spec
- Mate-pair sequencing
- Splice variant detection
The SageELF instrument includes a monitor, keyboard, and mouse. A CPU is built in; an external PC is not required.
|Sage Elf Specs|
|Electrophoresis voltage:||25V to 150V constant, adjustable, or pulsed field|
|Optical detector:||470 nm excitation | 525 nm emission|
|Power requirements:||100-240 VAC, 2.5 A, 50-60 Hz|
|Weight:||20 lbs / 9 kg|
|Dimensions||10.5h x 13.5w x 10.5d (in) | 27h x 34w x 27 d (cm)|
The SageELF has improved our workflows for both Illumina and PacBio sequencing by enabling precision size-selection of DNA libraries with minimal sample loss. The associated time savings have greatly improved both throughput and sequencing turnaround times.
John Innes Centre
Since acquiring the SageELF it has revolutionised the way we approach our NGS library construction. From day one the flexibility of the platform has been impressive and the global approach to size selections ensures minimal tweaking of experimental parameters to consistently achieve the best possible results for any given starting material. In the short time we have had the system we have improved the robustness of our Illumina based long mate pair protocol, it has enabled us to fractionate cDNA libraries to facilitate ISOseq on our PacBio RSII and allowed us to accurately recover the longest amplicons in RENseq applications. Whist its versatility has ensured that it has become a valuable asset within our lab the most exciting thing is that we have lots more potential applications on the horizon!
The Genome Analysis Centre (TGAC)