SensiQ Pioneer Biosensors (SPR)
We offer a wide range of surface chemistries to meet the growing needs of life science researchers. Protocols are available for all of these sensor surfaces enabling researchers to optimize their interaction analysis studies and ensure the generation of high quality data.
SensíQ Technologies' Recommendation for Kinetic Analysis Applications
We recommend two dimensional surface chemistry for kinetic applications as the interpretation of kinetic constants is simplified.
Gold Only Surface
Sensor provided with gold coated glass surface with no further modification to facilitate the attachment of user-developed functional chemistry.
The COOH1 surface is a two dimensional carboxylated coating suitable for a wide variety of biomolecular interactions. This surface has proven to resist non-specific binding better than any other coating by taking advantage of the chemical properties of polyethylene glycol (PEG). Coupling of ligands to this surface is normally achieved via amine coupling (EDC/NHS chemistry) but alternative methods such as thiol coupling, disulfide exchange and hydrazide coupling may also be employed.
The COOH2 surface is a short carboxylated polysaccaride coating suitable for a wide variety of biomolecular interactions. This surface is similar to COOH1 in charge potential but is more favorable in some applications as polysaccaride has different characteristics. The same coupling chemistries can be used with the COOH2 surface as with COOH1 surface.
The COOH5 surface is a hydrogel-based sensing surface that enables higher ligand capacity. It is composed of a biocompatible hydrophilic polysaccharide in a three-dimensional matrix. Carboxyl groups naturally present on the polysaccharide allow for ligands to be covalently linked via amine containing groups utilizing coupling techniques similar to those of the COOH1 surface. Ligands can be coupled in excess of 20kRU’s allowing binding interactions to be observed for small molecules and for ligands that exhibit low binding activity.
The BioCap surface is a two dimensional coating that utilizes the high affinity biotin – avidin interaction as the basis for ligand immobilization. A deglycosylated variant of avidin is covalently coupled to the BioCap surface; making it ideal for the capture of biotinylated reagents including peptides, oligonucleotides, proteins and sugars in a non-reversible manner.
The VesCap surface is a two dimensional chemistry for the immobilization of vesicles. Liposomes, unilamellar vesicles and even crude membrane preparations may be conveniently immobilized in a single step. A hydrocarbon chain is presented on this surface as an ‘anchor’ to hold the hydrophobic portion of the membrane to the surface. Components embedded in the vesicles will continue to diffuse and this property makes this surface ideal for membrane bound receptor studies.
The AvCap surface is a two dimensional coating utilizing biotin to capture avidin-conjugated ligands. It can reversibly bind a full monolayer of protein via the strong affinity that exists between biotin and the four binding sites of avidin, streptavidin and their derivatives. Avidin bound to a planar layer of biotin can be used to capture another biotinylated ligand using one of the other available sites. A solution of guanidine-HCl may be used to denature the protein leaving the surface-bound biotin residues free to recapture fresh conjugates.