The SageELF is a novel tool for fractionating DNA samples for next-gen sequencing. Featuring a unique electrophoretic design, the platform slices DNA samples into 12 contiguous fractions, and collects them in separate buffer-filled sample wells. The process is simplified into a few short steps: we provide the precast gel, software predicts the fractionation profile, and you then load your sample. After 2-3 hours, simply collect your fractions in buffer using a pipette.

Whole Sample Fractionation for NGS:
• Construct libraries with multiple insert sizes
• Pool fractions to optimize library size and amount
• Reduce complexity of total RNA samples
• Recover and archive valuable samples

Flexibility
Multiple-size NGS libraries from a single sample provide more flexibility in addressing sequencing challenges such as structural rearrangements, splice variant discovery, closing gaps, and understanding complex genomes.

Accuracy
Fraction sizes are typically within 15% of the software input value (within the range provided) when the internal standard is used.

High Sample Recovery
Between 50-80% of a sample can be recovered if the whole sample is fractionated.

Benefits of the SageELF System:                                       

• Reduces the complexity of RNA (cDNA) or protein samples
• Allows construction of DNA libraries with multiple insert sizes for improved assembly
• Allows sequencing of valuable DNA and retrieving sample for later study

Key Downstream Technologies:

• Proteomics and mass spec
• Mate-pair sequencing
• Splice variant detection

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Flexibility
Facilitates library construction for commonly used short-read NGS platforms, including Illumina (iSeq 100, MiniSeq, MiSeq, NextSeq, HiSeq, NovaSeq) and Ion Torrent

Rapid Run Times
Eliminates laborious, time-intensive steps associated with manual gels, thus reducing time from several hours to as little as 1 hour

Automated Programmable Runs
Eliminates intra and inter experimental variability associated with manual gel size selection

Pre-cast, Dye-free Disposable Agarose Gel Cassettes
Pre-cast agarose cassettes are free from SYBR and EtBr intercalating dyes, thus eliminating the need for subsequent sample clean-up

Ease-Of-Use
Offers highly intuitive software with pre-defined cassette conditions

Contaminant Removal
Eliminates primer-dimers and adaptor-dimers generated during PCR and ligation reaction, respectively

Downstream Application Compatibility
Compatible with a plethora of short-read NGS applications, including paired-end sequencing, emPCR, RNAseq and ChIPseq

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Flexibility
Facilitates library construction for commonly used short-read (including the Illumina iSeq 100, MiniSeq, MiSeq, NextSeq, HiSeq, NovaSeq, Ion Torrent PYRO 454) and long-read (Oxford Nanopore and PACBIO) NGS platforms.

Versatility
The integration of pulse-field capabilities enables the accurate size selection of DNA fragments from 100bp up to 50kb

Increased Compatibility
Sample elutes into a buffer-filled well that is compatible with all downstream applications (including NGS, PCR and ddPCR)

Minimal Physical Manipulation Required
Automation significantly reduces mechanical shearing and breakage of DNA, thus preserving large DNA fragment lengths for Oxford NanoPore and PACBIO workflows

Dye-free Agarose Gel Cassettes
Pre-cast cassettes are free from SYBER and EtBr interclating dyes, thus eliminating the need for subsequent sample clean-up

Eliminates Contamination
Sample Lanes within the pre-cast agarose cassettes are physically separated to eliminate cross-contamination between samples

Downstream Application Compatibility
Compatible with a plethora of applications, including paired-end sequencing, emPCR, RNAseq, ChiPseq, RADseq, mate-pair sequencing, single-molecule sequencing and bar-coded linked reads

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Pulsed-Field Technology
Utilises innovative pulsed field inversion electrophoresis that works by shuttling DNA back and forth in the gel, effectively slowing down large DNA fragments that would typically migrate at the same rate as smaller DNA fragments during conventional electrophoresis

Simple Plug-and-play Set-up
Simply load DNA samples, connect electrodes to Pippin Pulse, connect Pippin Pulse to computer via USB and select pre-configured programme

Flexible Programming
Customised protocols can be designed by simply editing parameters in the Pippin Pulse software (including run voltage, forward/reverse time steps and number of steps per cycle)

Rigorously Validated
Pre-set protocols have undergone extensive in-house validation by experienced Sage Science Application Scientists

Affordable
Experience the benefits of pulsed-field electrophoresis at an affordable price compared to other systems currently on the market

Cost-effective
No need to perform expensive long-read NGS runs to validate size selection efficiency. The Pippin Pulse can be used to QC DNA library quality before undertaking expensive NGS sequencing reactions, thus eliminating uncertainty

Robust Design
Midi gel box contains reinforced platinum electrodes for eliminating electrode corrosion during long runs.

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One-stop Sample Preparation
DNA extraction, enzymatic reaction and DNA size selection is undertaken in situ in agarose cassettes for long-read genomics

Flexible Enzymatic Treatments
A plethora of enzymatic treatments can be performed in situ (with endonucleases, ligases, nickases, cleavases or polymerases) to modify genomic DNA into labelled or tagged libraries

HLS-CATCH
Cas-9 Assisted Targeting of CHromosomal segments (CATCH) utilises guide RNAs (gRNAs) and a Cas9 nuclease that can be used for the targeted purification of HMW DNA

HMW DNA Target Enrichment
Enables analysis of pseudogenes segmental duplications and copy number variants

Fast run times
Rapid completion of runs:
Treatment (30 min)
Collection (1-6 hr)

Application Flexibility
Compatible with a plethora of downstream applications, including optical mapping, long-read sequencing, bar-coded linked reads and structural variant analysis

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High Throughput Capabilities
Building on the success of the Pippin Prep and Blue Pippin size selection technologies, the PippinHT provides the user with the ability to size select up to 24 samples

Sample Flexibility
Like the Blue Pippin, the PippinHT has pulsed field capabilities, thus enabling the size selection of DNA fragments intended for use in both short-read (including the Illumina iSeq 100, MiniSeq, MiSeq, NextSeq, HiSeq, NovaSeq, Ion Torrent PYRO 454) and long-read (Oxford Nanopore and PACBIO) NGS workflows

Proprietary Electro-elution Technology
samples are electro-eluted into a buffer-filled well that is compatible with all downstream applications, thus eliminating the need for further sample clean-up

High Sample Reproducibility
DNA fragments sizes and ranges are reproducibly collected, ensuring consistent results between runs

Downstream Application Compatibility
Compatible with a plethora of applications, including, RNAseq, ddRADseq, ChIPseq whole genome sequencing, miRNA library isolation, single molecule sequencing, Nanopore sequencing and droplet barcode sequencing

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