Blue Pippin

Automated DNA Size Selection for NGS Library Construction

The Sage Science product portfolio combines pre-cast agarose cassettes with a high-precision, automated solution for the accurate and targeted size-selection of DNA fragments for library construction in NGS workflows

Features & Benefits

  • Automated size selection
  • Eliminates operator-dependent variability
  • Short-read and long-read NGS library construction
  • Selects inserts from 100bp to 50kb
  • Eliminates contamination
  • Removal of adaptor dimers and PCR primer dimers
  • Compact design
  • Reduced footprint and saves valuable bench space

"At Earlham we work on a wide variety of different organisms and receive DNA samples from a number of different sources. One of the challenges that we face is that some samples contain a considerable number of DNA molecules that are smaller than the capability of the sequencing technology we want employ. These smaller molecules can often compromise the data outputs, reducing both yields and the spatial information they provide. The new High Pass Plus with its accurate size exclusion, shortened run time and improved recovery has allowed us to remove these smaller, DNA molecules helping us maximise the sequence yields and spatial information we generate and build more robust pipelines."

Darren Heavens , Earlham institute

Blue Sky thinking

Find out how Sage technology is helping the team at the Earlham Institute in Norwich improve their NGS data collection.

It has enabled us to develop a really robust mate pair protocol which we have recently published.

Darren Heavens
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Specifications

Instrument control

Built-in hard drive

Minimum sample amount/lane

15 ng

Maximum sample amount/lane

5 µg*

Electrophoresis voltage

25V, 100V or 150V constant, 100 V pulse field

Optical detection

470 nm excitation, 525 nm emission

Power requirements

100-250 VAC, 2.5 A, 50-60 Hz

Dimensions

7h x 11w x 21d (in), 18h x 28 w x53d (cm)

Weight

7. kg (15 lbs.)

* 10 µg when using the High Pass PLUS cassettes

Downloads

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